Sassari secondary unit

Area della Ricerca CNR, ISPA-Traversa la Crucca, 3 Loc. Baldinca, 07100 Sassari
E-mail: antonio.barberis@cnr.it
Phone: 079 2841710
Fax: 079 2841799

Staff members:

• 14 + 2 associates

Research Areas

• Postharvest pathophysiology of perishable products
  1. Reducing quality loss in fresh fruit and vegetable products from field to table
  2. Development of effective, low-cost, and eco-friendly treatment protocols to control the spread of biotic agents
• Fourth-range (minimally processed) product technologies
  1. Development of protocols to preserve the nutritional and visual quality of minimally processed products
  2. Cold chain optimization to extend shelf life
  3. Testing to determine optimal packaging characteristics for minimally processed fruit and vegetable products
  4. Real-time monitoring of quality using sensors and biosensors
• Recovery of phytocomplexes from waste and/or by-products derived from perishable raw materials or food processing
  1. Development of efficient and eco-sustainable extraction protocols
  2. Determination of anti-/pro-oxidant activities
  3. Chemical and electrochemical characterization of the extracts
• Diet and health: Bioactivity of phytocomplexes extracted from food matrices and gene expression of proteins in various cell lines
  1. Main cell lines studied: colon cancer, breast cancer, liver cancer, keratinocytes, fibroblasts
  2. Gene expression of proteins related to apoptosis and oxidative stress
  3. Enzymatic activities linked to oxidative stress responses

Laboratories and Major Equipment

• Preparative Chemistry Laboratory
  ASE Extractor – DIONEX 350
• Analytical Chemistry Laboratory
  HPLC
  GC-MS
• Postharvest Laboratory
  Temperature-, humidity-, and atmosphere-controlled chambers/cabinets
  Microbial growth chamber
  GC
  Fourth-range product processing line
  Penetrometer
  Colorimeter
• Cell Biology – Bioactivity Laboratory
  CO₂ incubator
  Mini CO₂-free CAPP incubator
  Microplate reader
  Fluorescence microscope
  Optical microscope connected to computer
  -80°C ultrafreezer
  
• Cell Biology – Gene Expression Laboratory
  Qubit 2.0 Fluorometer (Thermo Fisher Scientific)
  TProfessional Trio Thermocycler (Biometra)
  RotorGene Real-Time PCR (Qiagen)
  Western Blot system (Bio-Rad)
  3D orbital shaker
  Uvitech Mini HDS for chemiluminescence imaging
• Sensor Technology Laboratory
  Quadstat 4-channel potentiostat for amperometric sensors
  Stereoscope

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